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Mikio Kato, Ph.D.,
Professor
Faculty of Liberal
Arts and Sciences, Osaka Prefecture University, and Department of Biological Science, Graduate School of Science, Osaka
Prefecture University.
Research in the
Kato Laboratory comprises three areas. The first area involves biology of DNA
conformations. By utilizing atomic force- and electron-microscopes, we had
characterized the structure of intramolecular triplex DNA forming at the
specific DNA sequences existing at the eukaryotic genomes. Several types of
gbiasedh sequences have been isolated from the genomes and their conformational
preferences in vitro are being examined. We are also interested in RNA
secondary structures that relate to the ribozyme.
Second
area of interest involves understanding the molecular phylogeny of aquatic
organisms (both vertebrates and invertebrates). By sequencing and comparing
genomic DNA sequences, genetic relationships among various species and among
intraspecific populations can be evaluated. We have identified polymorphic
microsatellites from mayfly genomes that are useful to characterize the genetic
structures of subpopulations. We are currently conducting DNA barcoding of
aquatic insects (mayflies, stoneflies, and caddisflies) inhabiting Japan and
eastern Asian areas. Please visit J-ALOR home
(under construction).
Third
area involves understanding the biological effects of ionizing radiation.
Bacterial cells treated with accelerated ions or electromagnetic radiations may
have lost the potential of growth and division, but not all
of physiological activities have ceased immediately after the
treatments. Analyzing the physiological functions that remained after the
treatments will help investigate the functional structures of machineries. We
are currently focusing on the studies on bacterial motility after
irradiation.
Structures and function of nucleic acids
Mikio Kato, Jun Kudoh, Nobuyoshi Shimizu. The pyrimidine/purine-biased region of the epidermal growth factor receptor gene is sensitive to S1 nuclease and may form an intramolecular triplex. Biochem. J.(London) 268, 175-180 (1990).
Mikio Kato, Chad J. McAllister, Shingo Hokabe, Nobuyoshi Shimizu, Yuri L. Lyubchenko. Structural heterogeneity of pyrimidine/purine-biased DNA sequence detected by atomic force microscopy. Eur. J. Biochem. 269, 3632-3636 (2002).
Mikio Kato, Shingo Hokabe, Shuji Itakura, Shinsei Minoshima,Yuri
L. Lyubchenko, Theodor D. Gurkov,
Hiroshi Okawara, Kuniaki
Nagayama, Nobuyoshi Shimizu. Interarm interaction of
DNA cruciform forming at a short inverted repeat
sequence. Biophys.
J. 87, 402-408 (2003).
Nizar El-Murr,
Marie-Christine Maurel, Martina Rihova,
Jacques Vergne, Guy Hervé, Mikio Kato, Kunio
Kawamura. Behavior of a natural hammerhead ribozyme in aqueous solution at
medium to high temperatures with high salt conditions. Naturwissenschaften
99, 372-381 (2012).
Mikio
Kato. Biochemical
and microscopic analyses of DNA conformations. In: DNA Structure, Chromatin and Gene Expression
(2006) pp.17-26. ISBN: 81-7895-228-9 Editors: Ryoiti Kiyama and Mitsuhiro Shimizu.
Molecular phylogenetics and DNA taxonomy
Mikio Kato, Munetaka Ozeki, Akihiko Kikuchi, Toshio Kanbe. Phylogenetic relationship and mode of evolution of yeast DNA topoisomerase II gene in the pathogenic Candida species. Gene 272, 275-281 (2001).
Kei Wakimura, Yasuhiro Takemon, Atsushi Takayanagi, Shin-ichi Ishiwata, Kozo Watanabe, Kazumi Tanida, Nobuyoshi Shimizu, Mikio Kato. Characterization of genes for histone H3, 18S rRNA, and cytochrome oxidase subunit I of East Asian mayflies (Ephemeroptera). DNA Barcodes 4, 1-25 (2016).(archived in Portico)
Sharawy Z.Z., Abbas E.M., Khafage A.R., Galal-Khallaf A., Ismail R.F., Ahmed H.O., Mohammed-Geba K., Kato M. Descriptive analysis, DNA barcoding and condition index of Penaeids (Crustacea: Decapoda) from the Egyptian Mediterranean coast. Fisheries Res. 188, 6-16 (2017).
Kei Wakimura, Yasuhiro Takemon,
Shin-ichi Ishiwata, Kazumi
Tanida, Eman M. Abbas, Koji Inai,
Akikazu Taira, Aki Tanaka,
and Mikio Kato*. A reference
collection of Japanese aquatic macroinvertebrates. Ecological Genetics
and Genomics 17, 100065 (2020)
Koji Inai,
Kei Wakimura, and Mikio Kato*. Pairwise
sequence comparison data of the DNA barcodes of aquatic insects. Data in Brief 32, 106284 (2020)
Effects of ionizing radiation on
biological function
Mikio Kato, Walter Meissl, Kenji Umezawa, Tokihiro Ikeda, Yasunori
Yamazaki. Real-time observation of Escherichia coli cells under irradiation
with a 2-MeV H+ microbeam. Appl. Phys. Lett. 100, 193702 (2012). (*description in
Japanese๚{๊ษๆ้เeะ๎)
Tatsuo Atsumi,
Eriko Fujimoto, Masakazu Furuta, Mikio Kato. Effect
of gamma-ray irradiation on Escherichia coli motility. Cent. Eur.
J. Biol. 9, 909-914 (2014).
Mikio Kato. Effect
of ionizing radiation on the motility of Escherichia coli. In: "Microbes in the spotlight: recent progress in
the understanding of beneficial and harmful microorganisms" (2016) BrownWalker Press. ISBN-13: 978-1-62734-612-2. Editor: A.
Méndez-Vilas. pp.478-482.
Kei Wakimura, Yuta Okubo, Tatsuo Atsumi,
Mikio Kato. Loss of a negative chemotactic response in Escherichia coli after irradiation with gamma rays. In: "Exploring
Microorganisms: Recent Advances in Microbiology" (2018) BrownWalker Press. ISBN-13: 978-1-62734-623-8. Editor: A.
Méndez-Vilas. pp.281-285.